Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200557_ju86 CTGCTGGCATCACCACACTCAGCCAGCCCCTG(+CAGTGT)GATCCTTCTGACTTGGCTTTGCCATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605200555_ntv8 AAAACAAAAAAATGGCAAAGCCAAGTCAGAAG(+CAGTGT)GATCCAGGGGCTGGCTGAGTGTGGTGATGCC NGG 10 8 17 8 24 0 100 5 10
    240605200554_aeua AAGGAGGCAAGTCCTAACATGGGGGTGAGCTG(+CAGTGT)GATCCAGACACGGGTGGGTGCGTAGAGTGCC NGG 10 8 17 8 24 0 100 5 10
    240605200553_8ur5 CTCTCGGCACTCTACGCACCCACCCGTGTCTG(+CAGTGT)GATCCAGCTCACCCCCATGTTAGGACTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605200552_bl1r GGGGCAGGCCAAAAGATGGATGATAAGAAGTG(+CAGTGT)GATCCACTTGAGAGTGGCTGAAGAACCAAGG NGG 10 8 17 8 24 0 100 5 10
    240605200550_ndxp CGCTACCTTGGTTCTTCAGCCACTCTCAAGTG(+CAGTGT)GATCCACTTCTTATCATCCATCTTTTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605200549_151i CTTTAAGGATGTGTTAAAATCTGAAGGCATTG(+CAGTGT)GATCCTGAGGAATGATCAGGAGAGGCACTGA NGG 10 8 17 8 24 0 100 5 10
    240605200547_q1v2 CCTGCTCAGTGCCTCTCCTGATCATTCCTCAG(+CAGTGT)GATCCAATGCCTTCAGATTTTAACACATCCT NGG 10 8 17 8 24 0 100 5 10
    240605200546_o6fx AGCCGCGAACACCAAGAGGAAAGAAAAGGATG(+CAGTGT)GATCCCCCCCCCCAGTCCCCGTCTCCCATCC NGG 10 8 17 8 24 0 100 5 10
    240605200543_imoq GTGGGGGATGGGAGACGGGGACTGGGGGGGGG(+CAGTGT)GATCCATCCTTTTCTTTCCTCTTGGTGTTCG NGG 10 8 17 8 24 0 100 5 10