Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200525_con0 GCAGTTCCCACCTGTGGCCATGCTCAGCACTG(+CAGTGT)GTACCCAGCCGATTTTCAGTCAGGCTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605200524_t8qx AAAGGCAGAAAGCCTGACTGAAAATCGGCTGG(+CAGTGT)GTACCAGTGCTGAGCATGGCCACAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605200522_1gme CCTTGGGTTTCATTTAGCTTCTCACAGTCAAG(+CAGTGT)GATCCAAGACGCCAAATCTATGTGTGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605200521_mdkw GTCAAATGCTCACACATAGATTTGGCGTCTTG(+CAGTGT)GATCCTTGACTGTGAGAAGCTAAATGAAACC NGG 10 8 17 8 24 0 100 5 10
    240605200519_zfhq GTATTTAGAATGTTATATTTATTGTCGGTTTG(+CAGTGT)GTACCACCTTGTCCCAGGTGAGCATTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605200518_33i0 CCATTGGCCAAATGCTCACCTGGGACAAGGTG(+CAGTGT)GTACCAAACCGACAATAAATATAACATTCTA NGG 10 8 17 8 24 0 100 5 10
    240605200516_kh9w AAAGAGGGGGAGGAATGAGACAGGACAGGCAG(+CAGTGT)GATCCCTGAAATAACTGGAACGCACAATAAG NGG 10 8 17 8 24 0 100 5 10
    240605200514_td4h GCCATCTTATTGTGCGTTCCAGTTATTTCAGG(+CAGTGT)GATCCTGCCTGTCCTGTCTCATTCCTCCCCC NGG 10 8 17 8 24 0 100 5 10
    240605200513_i635 GAGTGTCAGGACTCTGAAGGAAGGGCAGCCAG(+CAGTGT)GTACCAAGCTACCTCCTTCCCACCCTCAACC NGG 10 8 17 8 24 0 100 5 10
    240605200511_a662 AGTTGGGTTGAGGGTGGGAAGGAGGTAGCTTG(+CAGTGT)GTACCTGGCTGCCCTTCCTTCAGAGTCCTGA NGG 10 8 17 8 24 0 100 5 10