Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200616_xlo7 GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+CAGTGT)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605200615_zktb ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+CAGTGT)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605200613_qkhq GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+CAGTGT)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605200612_na7b ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+CAGTGT)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605200609_qmwn GAACGCCTACTTCCTGTCTCTGCTGTCTCCCG(+CAGTGT)GTACCTGTGCTTCTGAGCCCAGACTATCTCT NGG 10 8 17 8 24 0 100 5 10
    240605200608_3ms0 TGACAAGAGATAGTCTGGGCTCAGAAGCACAG(+CAGTGT)GTACCGGGAGACAGCAGAGACAGGAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240605200605_ubr7 AGCAGGATGCAAATGTGGAGGCAGTCCCAGAG(+CAGTGT)GATCCCAAAACTGCTGCCAAGTTACAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605200604_d0qe GGGTGTCTCCTGTAACTTGGCAGCAGTTTTGG(+CAGTGT)GATCCTCTGGGACTGCCTCCACATTTGCATC NGG 10 8 17 8 24 0 100 5 10
    240605200602_swk8 GGACAATCCTGACCCTCTTCCGCTGGCCTGTG(+CAGTGT)GATCCCTTGACAAGCGACTCTGTTCTGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605200600_8q1o GACCCTGGACAGAACAGAGTCGCTTGTCAAGG(+CAGTGT)GATCCACAGGCCAGCGGAAGAGGGTCAGGAT NGG 10 8 17 8 24 0 100 5 10