Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200423_o78n CACACACACCAGGGTGAAATGTAAAGAAACAG(+CAGTGT)GATCCTGGCACTAAGATTGCATAGCCCATCA NGG 10 8 17 8 24 0 100 5 10
    240605200421_xywk TGAGATGATGGGCTATGCAATCTTAGTGCCAG(+CAGTGT)GATCCTGTTTCTTTACATTTCACCCTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605200420_vkmk ACAGCAACAGAAGCCCTAACTAAGACAAGTTG(+CAGTGT)GTACCAGTGCAGTGGGATGTTGACTCGACAC NGG 10 8 17 8 24 0 100 5 10
    240605200418_82uw CAGGTGTGTCGAGTCAACATCCCACTGCACTG(+CAGTGT)GTACCAACTTGTCTTAGTTAGGGCTTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240605200416_q394 CAGGAGGAGCAGAGGGTTTGAGATGATGGTGG(+CAGTGT)GTACCATCAACCCACACTCACCTCTCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605200414_8439 AGGCTTGCCAGAGAGGTGAGTGTGGGTTGATG(+CAGTGT)GTACCCACCATCATCTCAAACCCTCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605200413_rrq1 CTTTCTTTCTTTTTCTCCTTACTTATTTTGTG(+CAGTGT)GTACCTGAGACTAAACCCAGGGCCTCCCATG NGG 10 8 17 8 24 0 100 5 10
    240605200412_lvvx AGGCACATGGGAGGCCCTGGGTTTAGTCTCAG(+CAGTGT)GTACCACAAAATAAGTAAGGAGAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605200409_6l6d ACATCGGGAGAACTCTGAATGTTGTTCACAGG(+CAGTGT)GATCCTTAGGACACAGCTCCTCTGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605200407_u2n5 AGTTTATAGAAACAGAGGAGCTGTGTCCTAAG(+CAGTGT)GATCCCTGTGAACAACATTCAGAGTTCTCCC NGG 10 8 17 8 24 0 100 5 10