Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200406_fqwr AGCTCAGTGATAGCACTTGCCTCGGGTGCACG(+CAGTGT)GTACCCTGGTTTAGATGCCCTGCACTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605200404_hxe8 ATTTTCTTTCAGTGCAGGGCATCTAAACCAGG(+CAGTGT)GTACCGTGCACCCGAGGCAAGTGCTATCACT NGG 10 8 17 8 24 0 100 5 10
    240605200403_7srs CTTAATCAGCTCACCCGCTCACAGGGTATCTG(+CAGTGT)GTACCTTAACTCCTGCCGGGTCTGAGTATTG NGG 10 8 17 8 24 0 100 5 10
    240605200401_y1gp ATTTACAATACTCAGACCCGGCAGGAGTTAAG(+CAGTGT)GTACCAGATACCCTGTGAGCGGGTGAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605200400_u4jf ACCTCATACAAAACTTGTTCAACAGTCTAGTG(+CAGTGT)GTACCAACAGCGTTGAGATGGCAGTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240605200358_t0b5 TGTAGCGCAAAACTGCCATCTCAACGCTGTTG(+CAGTGT)GTACCACTAGACTGTTGAACAAGTTTTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605200357_v5lt TGATCATTATGACAGGTCGCTGCCCTCCAGTG(+CAGTGT)GTACCACTGACCTACAGAAGGACATCATGGC NGG 10 8 17 8 24 0 100 5 10
    240605200355_cszi GCTCTGCCATGATGTCCTTCTGTAGGTCAGTG(+CAGTGT)GTACCACTGGAGGGCAGCGACCTGTCATAAT NGG 10 8 17 8 24 0 100 5 10
    240605200354_d1dq GCAGCTCAGGGTTGGGGCCTAGTTACAGTTTG(+CAGTGT)GATCCTTAGTCCAAGAATTTTGAGAACGTGA NGG 10 8 17 8 24 0 100 5 10
    240605200353_e5t8 TTTACTCACGTTCTCAAAATTCTTGGACTAAG(+CAGTGT)GATCCAAACTGTAACTAGGCCCCAACCCTGA NGG 10 8 17 8 24 0 100 5 10