Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200300_y9jf AACCACAGCTCTGCTAGTCAGGACAACGTTAG(+CAGTGT)GTACCACTGACGACCCACAGGCCACTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605200258_agxi ACTCGGCAGGAGTGGCCTGTGGGTCGTCAGTG(+CAGTGT)GTACCTAACGTTGTCCTGACTAGCAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605200257_72iv ATGTTGATTTAGGTATACACACTATAGAATGG(+CAGTGT)GATCCAAGATCTTTAACAGCTTGCTTGTATT NGG 10 8 17 8 24 0 100 5 10
    240605200255_h6fx AAAAAAATACAAGCAAGCTGTTAAAGATCTTG(+CAGTGT)GATCCCATTCTATAGTGTGTATACCTAAATC NGG 10 8 17 8 24 0 100 5 10
    240605200252_1bn3 GTAGGCGCGCTGGCCCGGCCACCGACACCGGG(+CAGTGT)GATCCCCGCTGCAGCGCCCAAACCGTGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605200250_q2gx GCTAGAGGACACGGTTTGGGCGCTGCAGCGGG(+CAGTGT)GATCCCCGGTGTCGGTGGCCGGGCCAGCGCG NGG 10 8 17 8 24 0 100 5 10
    240605200249_l13r TTATCTTCACTTCCCCAGGAAGCAGTGACTGG(+CAGTGT)GATCCTTTGGGCATCGCCACTTTTGGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605200246_1r04 GTAACATCTTCCAAAAGTGGCGATGCCCAAAG(+CAGTGT)GATCCCAGTCACTGCTTCCTGGGGAAGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605200245_kllf ATATTAAAAAACAGAAGTGGCAATTCAGTAAG(+CAGTGT)GATCCTTCCATTGTTCTTTATACAGATTTAA NGG 10 8 17 8 24 0 100 5 10
    240605200244_1o6j AGCATTTAAATCTGTATAAAGAACAATGGAAG(+CAGTGT)GATCCTTACTGAATTGCCACTTCTGTTTTTT NGG 10 8 17 8 24 0 100 5 10