Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200152_v4wj GAGCTGTTAGCTACCTCTAAGGCTCCCATTGG(+CAGTGT)GATCCTGTGCTTTTTATTTACAGTAAAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605200150_h631 GACAAATGCTTTACTGTAAATAAAAAGCACAG(+CAGTGT)GATCCCAATGGGAGCCTTAGAGGTAGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240605200148_n4rn CTGGATGCTTCACTCCTGTACAAGTCCCGTTG(+CAGTGT)GTACCATATTCGAATGTTATTCTCTTCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605200147_txdf GTAAACAGAGAAGAGAATAACATTCGAATATG(+CAGTGT)GTACCAACGGGACTTGTACAGGAGTGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605200145_ihyk ACTTATTAACAAGCCGAGCAGAATTTTTATAG(+CAGTGT)GATCCATCCATCCAGATTTATTACAGAACAC NGG 10 8 17 8 24 0 100 5 10
    240605200144_1pfb AAATGGTGTTCTGTAATAAATCTGGATGGATG(+CAGTGT)GATCCTATAAAAATTCTGCTCGGCTTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605200142_tc5a TCTTGAGTTGATGATAACTGGAAGTCCACCTG(+CAGTGT)GTACCTGCCTTCCTGTTCATTTGTCTTCATT NGG 10 8 17 8 24 0 100 5 10
    240605200140_cser CTTTCAATGAAGACAAATGAACAGGAAGGCAG(+CAGTGT)GTACCAGGTGGACTTCCAGTTATCATCAACT NGG 10 8 17 8 24 0 100 5 10
    240605200138_l40h ATAGTAAAGTCTAAAAATGAGCAAGCCATTGG(+CAGTGT)GTACCAACAGGTTCAAATGTTAGCTTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605200137_8djw ACACCACTGCAAGCTAACATTTGAACCTGTTG(+CAGTGT)GTACCCAATGGCTTGCTCATTTTTAGACTTT NGG 10 8 17 8 24 0 100 5 10