Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200047_72hr TAGAAAATTGTTAGCTGTCGTGTGGATGCTGG(+CAGTGT)GATCCCAGATCCTCAGCGCTCTTCATCACTG NGG 10 8 17 8 24 0 100 5 10
    240605200045_wg52 TGGCTCAGTGATGAAGAGCGCTGAGGATCTGG(+CAGTGT)GATCCCAGCATCCACACGACAGCTAACAATT NGG 10 8 17 8 24 0 100 5 10
    240605200043_rzvw TGGCCTGTGGGAATTATACACTCTACAACCAG(+CAGTGT)GATCCAAGCATCACAGATGCAGGAGATCCCA NGG 10 8 17 8 24 0 100 5 10
    240605200041_7t6o TGGCTTGGGATCTCCTGCATCTGTGATGCTTG(+CAGTGT)GATCCTGGTTGTAGAGTGTATAATTCCCACA NGG 10 8 17 8 24 0 100 5 10
    240605200040_exec CCTGATACTCACAGAGGCCAGAAGAATGTACG(+CAGTGT)GATCCCCAGTAACAGGAGTTGCAGATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605200038_8k3z ATGGCTCACAATCTGCAACTCCTGTTACTGGG(+CAGTGT)GATCCGTACATTCTTCTGGCCTCTGTGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605200036_29gy TCCTCAGAAAATTGGAAATAGTACTACTGGAG(+CAGTGT)GATCCTGCAATACCTCTCAGCAGTTTTGAGT NGG 10 8 17 8 24 0 100 5 10
    240605200034_ak3u ATACTACTCAAAACTGCTGAGAGGTATTGCAG(+CAGTGT)GATCCTCCAGTAGTACTATTTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605200032_uazg CTATAAAATGAGGTATGTGAGGAGGACTGTGG(+CAGTGT)GTACCTGGGCACAAGATTTCTGAAACCGGTA NGG 10 8 17 8 24 0 100 5 10
    240605200029_4ns9 GTGGATACCGGTTTCAGAAATCTTGTGCCCAG(+CAGTGT)GTACCCACAGTCCTCCTCACATACCTCATTT NGG 10 8 17 8 24 0 100 5 10