Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200028_dlyz ATGACACTGTCCAGAGATGTCCTCCCAGACTG(+CAGTGT)GTACCTTCGTAAACACACAAGCAGGGTACAA NGG 10 8 17 8 24 0 100 5 10
    240605200027_wq6i GGCTTTTGTACCCTGCTTGTGTGTTTACGAAG(+CAGTGT)GTACCAGTCTGGGAGGACATCTCTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240605200025_vz24 ACAATTTGGGCAGTGCCATTCCTTAGGCTTGG(+CAGTGT)GTACCAGGCTGCACAATAGCACAGAGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240605200023_l0nv TCATCTAAAGCTCTGTGCTATTGTGCAGCCTG(+CAGTGT)GTACCCAAGCCTAAGGAATGGCACTGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240605200021_fg32 TTGACATTAGGGAACTAAGTTGAGAGATTGAG(+CAGTGT)GATCCCCAAATCAACAACCAAGAAAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605200019_lghu TCCTTCAGAATTTTCTTGGTTGTTGATTTGGG(+CAGTGT)GATCCTCAATCTCTCAACTTAGTTCCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240605200017_rmd0 TAGAAGTGACCTGGAGGGAGTCTTCACAGAGG(+CAGTGT)GATCCTCTAGATGAAGTTCTCCTGTGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605200016_3gzk TAGACAGGCTCACAGGAGAACTTCATCTAGAG(+CAGTGT)GATCCCTCTGTGAAGACTCCCTCCAGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240605200014_t7n1 AGCAACAATGAACCACTGCCCTCATTGTCAGG(+CAGTGT)GATCCACGACTTTCCTGGCCTTGCAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605200013_2gpj AAGCTCATGCTTGCAAGGCCAGGAAAGTCGTG(+CAGTGT)GATCCCTGACAATGAGGGCAGTGGTTCATTG NGG 10 8 17 8 24 0 100 5 10