Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200102_ei9k AATTTTTCTTTATCTCCTCTTTAAATGCCCTG(+CAGTGT)GTACCTCAGCATCAGAGCCTCTCCCAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605200101_wu61 AGCTGTCAGCTGGGAGAGGCTCTGATGCTGAG(+CAGTGT)GTACCAGGGCATTTAAAGAGGAGATAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605200100_igyy CACAGGATAAATCCATGGTAATTCCTATAAAG(+CAGTGT)GTACCTACTGCTCCCATGAGCCAGCCCGAAA NGG 10 8 17 8 24 0 100 5 10
    240605200058_1rwj ACAGATTTCGGGCTGGCTCATGGGAGCAGTAG(+CAGTGT)GTACCTTTATAGGAATTACCATGGATTTATC NGG 10 8 17 8 24 0 100 5 10
    240605200057_tcns AAATCCCAGCACCGGGAAAGAGGAGCCAGGAG(+CAGTGT)GATCCTCTGGAGCCTGTTGGCAAGTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605200055_8yke TAGCTAGAAAGACTTGCCAACAGGCTCCAGAG(+CAGTGT)GATCCTCCTGGCTCCTCTTTCCCGGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605200053_aod0 CCCTTGAGGGGCGCACCCCCCAAAGGCTCCCG(+CAGTGT)GATCCCCTAAAGTTTCACAGCACCTCTGACT NGG 10 8 17 8 24 0 100 5 10
    240605200051_nmd6 GTACAAGTCAGAGGTGCTGTGAAACTTTAGGG(+CAGTGT)GATCCGGGAGCCTTTGGGGGGTGCGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605200050_5smb TTTAAAAGTGCAAAGTCTCTCTGCAAATGGTG(+CAGTGT)GATCCTGTATGGGATAGTCCCCTTCTCATAG NGG 10 8 17 8 24 0 100 5 10
    240605200048_sze1 AACTCCTATGAGAAGGGGACTATCCCATACAG(+CAGTGT)GATCCACCATTTGCAGAGAGACTTTGCACTT NGG 10 8 17 8 24 0 100 5 10