Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195837_bfob GGTAATCCTGTATTTCAGGGAAGTAGGAGGTG(+CAGTGT)GTACCTAAGGATGACTTATGAAGGATTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605195836_bqjk GCTAACTGACCAAGCTTCTCCCGCTCGTGCAG(+CAGTGT)GTACCTCCCTGATGGGAACACCAGAGACTGC NGG 10 8 17 8 24 0 100 5 10
    240605195834_8gze GAGCAGCAGTCTCTGGTGTTCCCATCAGGGAG(+CAGTGT)GTACCTGCACGAGCGGGAGAAGCTTGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605195832_a9af GGAAGAAAGACTGAGACCATCTGCTTTGGATG(+CAGTGT)GATCCATTAGCAAGCCTGGTCTGACTACACA NGG 10 8 17 8 24 0 100 5 10
    240605195830_7mpo GCCCCTGTGTAGTCAGACCAGGCTTGCTAATG(+CAGTGT)GATCCATCCAAAGCAGATGGTCTCAGTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605195829_9jqy CTTTGTGGTAAGTCTTTGCTTCAGACTGTTGG(+CAGTGT)GTACCCAGAACGAGAGCTATAAAAATGCATT NGG 10 8 17 8 24 0 100 5 10
    240605195826_q284 TGAACAATGCATTTTTATAGCTCTCGTTCTGG(+CAGTGT)GTACCCAACAGTCTGAAGCAAAGACTTACCA NGG 10 8 17 8 24 0 100 5 10
    240605195825_cp66 CTTTATTTGGATAGCAGCGCTTCAGTTCCTAG(+CAGTGT)GATCCTTTTATAAATGGTAAAACCCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240605195823_h4cd TTGTAAATCCAGGGTTTTACCATTTATAAAAG(+CAGTGT)GATCCTAGGAACTGAAGCGCTGCTATCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605195821_n16a CTGACAAAGAGGTGGGCCAGGATGGGACATGG(+CAGTGT)GTACCAGGAGTGCACAGGCTTGGGCAATTTG NGG 10 8 17 8 24 0 100 5 10