Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195949_nsrc TTGCTAGAGGAAAAATAGACAGAGAATCTAAG(+CAGTGT)GTACCCCTGTCCCCAGAGCATGGTCATTTCC NGG 10 8 17 8 24 0 100 5 10
    240605195947_oekb CCCGAGGAAATGACCATGCTCTGGGGACAGGG(+CAGTGT)GTACCTTAGATTCTCTGTCTATTTTTCCTCT NGG 10 8 17 8 24 0 100 5 10
    240605195945_cb4f ACATCCACCATATGCCTGCTCCCCTGTCGCCG(+CAGTGT)GTACCAAGTGAGATGTCTGTGTGGCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605195944_j2xf TGCAAAAACAAGCCACACAGACATCTCACTTG(+CAGTGT)GTACCGGCGACAGGGGAGCAGGCATATGGTG NGG 10 8 17 8 24 0 100 5 10
    240605195942_ve5l TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+CAGTGT)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605195941_wmfx TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+CAGTGT)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605195940_ujmm TTTTGAGGACTCTTAATAAAAATTTTTGAGTG(+CAGTGT)GTACCTAAGTTTAGGTTTAGAGCACTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605195938_q7qm TTATTGGCAGAGTGCTCTAAACCTAAACTTAG(+CAGTGT)GTACCACTCAAAAATTTTTATTAAGAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605195937_6olx TTTAGCCTTTGTGGGAAAGAGTAAGGCATGTG(+CAGTGT)GATCCGACCAGGTGGTAAGGTTGTAAGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605195935_kum0 GACAAGTTCCTTACAACCTTACCACCTGGTCG(+CAGTGT)GATCCACATGCCTTACTCTTTCCCACAAAGG NGG 10 8 17 8 24 0 100 5 10