Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200137_8djw ACACCACTGCAAGCTAACATTTGAACCTGTTG(+CAGTGT)GTACCCAATGGCTTGCTCATTTTTAGACTTT NGG 10 8 17 8 24 0 100 5 10
    240605200135_8crz GCTTCCCTGCCAGCCCGCATAGGAGGTGTGTG(+CAGTGT)GATCCTAATAGGTTGAAGTTTTTAAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240605200134_0f5o TCCTCCATGCTTTAAAAACTTCAACCTATTAG(+CAGTGT)GATCCACACACCTCCTATGCGGGCTGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605200132_9pnm AGTCTCCTGAGTACTGGGTTTGCAGGTTTCGG(+CAGTGT)GTACCACATCAGCTTGATTTCATAATGGATA NGG 10 8 17 8 24 0 100 5 10
    240605200130_3ge3 TCCTTTATCCATTATGAAATCAAGCTGATGTG(+CAGTGT)GTACCCGAAACCTGCAAACCCAGTACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605200129_pzb6 AGCAGTATGCATAAAATCAAAGATCTACTCAG(+CAGTGT)GATCCCTTACAACAGAAAACCACCAAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605200127_qh52 TGGCAAGTTTTTGGTGGTTTTCTGTTGTAAGG(+CAGTGT)GATCCTGAGTAGATCTTTGATTTTATGCATA NGG 10 8 17 8 24 0 100 5 10
    240605200125_bxx5 AAGAATGCATTAAAGATGAACTTGATCGTTGG(+CAGTGT)GTACCTAGTGTATCCTAGGCAGGATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605200124_0qq8 TAAATAGCAGCATCCTGCCTAGGATACACTAG(+CAGTGT)GTACCCAACGATCAAGTTCATCTTTAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605200122_dd2j ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCTATGCCCTCTTCTGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10