Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194707_0aa6 TTGAGTTTCTCAAAATATAATATTTATTGTTG(+CAGTGT)GATCCATTATCTTAAAAGAATCATCCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605194706_1gcp AGTCTTCCCAGGATGATTCTTTTAAGATAATG(+CAGTGT)GATCCAACAATAAATATTATATTTTGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605194703_agzw ACCTGTCACACACCTCCACCCCAAGGTTAAGG(+CAGTGT)GATCCAGGATGAAGACTCTAAGAGCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605194701_dsxe CATTGCTTCTGGCTCTTAGAGTCTTCATCCTG(+CAGTGT)GATCCCTTAACCTTGGGGTGGAGGTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605194700_dk78 ATATGTACGCACAGGGCCATGAACGAGGTGAG(+CAGTGT)GTACCAGCTTCTTCACAGATTCCATACTTGT NGG 10 8 17 8 24 0 100 5 10
    240605194659_6bnr AGTACACAAGTATGGAATCTGTGAAGAAGCTG(+CAGTGT)GTACCTCACCTCGTTCATGGCCCTGTGCGTA NGG 10 8 17 8 24 0 100 5 10
    240605194657_4x93 ATATAGTATAAATGTTTTTATGTACCAGTGAG(+CAGTGT)GTACCAGTGTCTCTCTGGAGGTTGGATCTGT NGG 10 8 17 8 24 0 100 5 10
    240605194656_nph1 AGACCACAGATCCAACCTCCAGAGAGACACTG(+CAGTGT)GTACCTCACTGGTACATAAAAACATTTATAC NGG 10 8 17 8 24 0 100 5 10
    240605194655_7gu0 CACCATACCTGTTTTATGTGTGTCTCCTTCTG(+CAGTGT)GATCCTCTCTCGGTGTGGTTGCCACTACCAC NGG 10 8 17 8 24 0 100 5 10
    240605194653_gz4z TCCAAGTGGTAGTGGCAACCACACCGAGAGAG(+CAGTGT)GATCCAGAAGGAGACACACATAAAACAGGTA NGG 10 8 17 8 24 0 100 5 10