Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194743_tf7j GAGGAGACAGAAAAGGCAGTGGTGTCCACTAG(+CAGTGT)GATCCTCTGTCCACCACTTGAGATAAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605194742_u52o ACACCGTACCTTATCTCAAGTGGTGGACAGAG(+CAGTGT)GATCCTAGTGGACACCACTGCCTTTTCTGTC NGG 10 8 17 8 24 0 100 5 10
    240605194739_a1sn CATTTGCAGAGTGGACCCAGCAGTGCCCACAG(+CAGTGT)GATCCTTATTCACGTATCTAAGTCTGATATT NGG 10 8 17 8 24 0 100 5 10
    240605194737_g460 CATAGAATATCAGACTTAGATACGTGAATAAG(+CAGTGT)GATCCTGTGGGCACTGCTGGGTCCACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605194735_j1v9 AGTAGGACCATCCAGAAACTTCTTCACCCAGG(+CAGTGT)GATCCATCCCATAGTCAGCCACCAAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240605194733_7zfo AGTGTCTGCATTTGGTGGCTGACTATGGGATG(+CAGTGT)GATCCCTGGGTGAAGAAGTTTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605194732_xyy5 CAGTGAAGTTCAGTCAAGTCAAAGGTCAATTG(+CAGTGT)GATCCCAATGTGGCTGCCTGAAAGGTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240605194729_sp19 TGTGGTAACAACCTTTCAGGCAGCCACATTGG(+CAGTGT)GATCCAATTGACCTTTGACTTGACTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240605194728_ba6j TATCTGTTTAGTCAAAACTCTGAGTGTGCTTG(+CAGTGT)GATCCCTCTGTTTGATCTGTCAAACTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605194726_5gnr ATGTGGGAAGAGTTTGACAGATCAAACAGAGG(+CAGTGT)GATCCAAGCACACTCAGAGTTTTGACTAAAC NGG 10 8 17 8 24 0 100 5 10