Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194852_a3ze TCCAGGGTATCTAACACTTCTGGCCTCCACAG(+CAGTGT)GTACCTGTCTCACAGGACCATAACCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605194851_8l13 TTTCTCTGTGGGGTTATGGTCCTGTGAGACAG(+CAGTGT)GTACCTGTGGAGGCCAGAAGTGTTAGATACC NGG 10 8 17 8 24 0 100 5 10
    240605194849_630s TTGTGACAAATATTTTTGCCTTTCAAAGTTAG(+CAGTGT)GTACCAGAAATATCAAGACTTGGGTTAACCA NGG 10 8 17 8 24 0 100 5 10
    240605194848_c741 TATCATGGTTAACCCAAGTCTTGATATTTCTG(+CAGTGT)GTACCTAACTTTGAAAGGCAAAAATATTTGT NGG 10 8 17 8 24 0 100 5 10
    240605194846_0upu AATCCACAGAGGATTTCACATGATGTCAGCCG(+CAGTGT)GTACCGGGAGGAGCCATGCAGGATGGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605194843_xzcb GACAAGTGCACCATCCTGCATGGCTCCTCCCG(+CAGTGT)GTACCGGCTGACATCATGTGAAATCCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605194841_7kp6 CCATGCAGGATGGTGCACTTGTCTGTGAGTTG(+CAGTGT)GATCCTTAAAACTACCTAGAGGAACTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605194840_l7ot AGATGGCAGAAGTTCCTCTAGGTAGTTTTAAG(+CAGTGT)GATCCAACTCACAGACAAGTGCACCATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605194839_4otm ATCTGGGTATATACCCAGGATTGGTATTGCTG(+CAGTGT)GATCCTCCGGGTAGTACTATGTCCAATTTTC NGG 10 8 17 8 24 0 100 5 10
    240605194836_dtqh CCTCAGAAAATTGGACATAGTACTACCCGGAG(+CAGTGT)GATCCAGCAATACCAATCCTGGGTATATACC NGG 10 8 17 8 24 0 100 5 10