Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194927_dcud ACCCCCAGGAGAGAGTGGTGGAGAGAGTGGAG(+CAGTGT)GATCCCGCAGGTCTTTGCCAATTAAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605194925_836w TACCCCAAAGTTTAATTGGCAAAGACCTGCGG(+CAGTGT)GATCCTCCACTCTCTCCACCACTCTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605194921_wtsl AGAAAACAGCCTGGAAGGGTAAGTACCCCCGG(+CAGTGT)GTACCATACACTTCTGAAATCCCGAGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605194919_7mz3 GCCCCAAAGGCTCGGGATTTCAGAAGTGTATG(+CAGTGT)GTACCCGGGGGTACTTACCCTTCCAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605194918_vltb CTCAGCCTTCCACGTGCTGTGATTATAGGCAG(+CAGTGT)GTACCACCATATACAGCATATTGTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605194916_3st5 TTATAGACACATACAATATGCTGTATATGGTG(+CAGTGT)GTACCTGCCTATAATCACAGCACGTGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605194915_g4rz AGGTCCTCCTGCTTCAGCTTCTTACACAGAGG(+CAGTGT)GATCCCAGAGTGTGCCACCACCGCCAGCGTC NGG 10 8 17 8 24 0 100 5 10
    240605194913_bpy0 GCGGTGACGCTGGCGGTGGTGGCACACTCTGG(+CAGTGT)GATCCCTCTGTGTAAGAAGCTGAAGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605194911_4hen CTTCCAGCAGGAGACCCCGCCCTCACTGGGTG(+CAGTGT)GATCCTTCTTTAGTATCACGTGCTCACACTG NGG 10 8 17 8 24 0 100 5 10
    240605194910_85y3 CAAGCCAGTGTGAGCACGTGATACTAAAGAAG(+CAGTGT)GATCCACCCAGTGAGGGCGGGGTCTCCTGCT NGG 10 8 17 8 24 0 100 5 10