Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195015_flft CATGAGCAAACTTTTGAAACATCTTCAAGAAG(+CAGTGT)GTACCAAGGGATTGCAGTGCATAATTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605195012_uwgq TTACAGCCAAAATTATGCACTGCAATCCCTTG(+CAGTGT)GTACCTTCTTGAAGATGTTTCAAAAGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240605195011_2smz TCTGACCCACATCAAGCCTCAGACGACAGATG(+CAGTGT)GTACCTGCCTGTTAAAGCATAACAGGGTGAT NGG 10 8 17 8 24 0 100 5 10
    240605195009_4ccu CACCCATCACCCTGTTATGCTTTAACAGGCAG(+CAGTGT)GTACCATCTGTCGTCTGAGGCTTGATGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605195008_l45u ACTCCCCACTGGCATAAGGTGCCGGTCTGTGG(+CAGTGT)GTACCATCCGAGCTCCTGAGCAATGCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605195006_hmrs TTGGGACTGAGCATTGCTCAGGAGCTCGGATG(+CAGTGT)GTACCCACAGACCGGCACCTTATGCCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605195004_lxpq TACCGCTTTCTCTGAGCACTCTGGGTGGCCAG(+CAGTGT)GTACCATCAACGTTTTGATCCAATACCTATG NGG 10 8 17 8 24 0 100 5 10
    240605195003_w71e GCTGTCATAGGTATTGGATCAAAACGTTGATG(+CAGTGT)GTACCTGGCCACCCAGAGTGCTCAGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605195002_qmea AACTTGGCAATAGCCAAGTCTAGAAGATGCTG(+CAGTGT)GTACCAAATAGCCTAATTCTTGTGATTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605195001_higf GGACTCTCCAATCACAAGAATTAGGCTATTTG(+CAGTGT)GTACCAGCATCTTCTAGACTTGGCTATTGCC NGG 10 8 17 8 24 0 100 5 10