Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194959_5k05 TGGAACAAAGTCATAACCTTGTCATTGAAGAG(+CAGTGT)GTACCAGAACTGAATTGAGACAAGAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240605194958_tpdi CCAAATATTTCTCTTGTCTCAATTCAGTTCTG(+CAGTGT)GTACCTCTTCAATGACAAGGTTATGACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605194956_cxaf GGGAGCAGAAAAAAATGGTCAGGGCTCTGATG(+CAGTGT)GATCCAAACTTGGCTGTGCCCACTGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605194955_jdw4 TGATGAGACAACAGTGGGCACAGCCAAGTTTG(+CAGTGT)GATCCATCAGAGCCCTGACCATTTTTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605194954_je02 CTTTTCCTGCTTTAAAATTCAGACTGTAACAG(+CAGTGT)GATCCCTTTCTAAAGGAATCTGTACATTCAT NGG 10 8 17 8 24 0 100 5 10
    240605194952_px5x AAAGCATGAATGTACAGATTCCTTTAGAAAGG(+CAGTGT)GATCCTGTTACAGTCTGAATTTTAAAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605194951_u3rx CTCTTTGTAGCTGAGGGTGACTTTAAACTTCG(+CAGTGT)GATCCTCCTGCTTACCCACCCCCAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240605194949_3qbx AATCCCAGCACTTGGGGGTGGGTAAGCAGGAG(+CAGTGT)GATCCGAAGTTTAAAGTCACCCTCAGCTACA NGG 10 8 17 8 24 0 100 5 10
    240605194947_v9gd TGGGTAGAGATTTGGTCCAGCGCATTCTCATG(+CAGTGT)GATCCTTCTCATCTTTTCCCAGGAACACTCG NGG 10 8 17 8 24 0 100 5 10
    240605194946_hg3p GGGAGCGAGTGTTCCTGGGAAAAGATGAGAAG(+CAGTGT)GATCCATGAGAATGCGCTGGACCAAATCTCT NGG 10 8 17 8 24 0 100 5 10