Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195104_vs9x ACTGGAGCCAGGGTCCTCCGCACTGACTTCCG(+CAGTGT)GATCCATTTGCTAAGTTGCCTGCTGTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605195102_1egm GACCTGGACAACAGCAGGCAACTTAGCAAATG(+CAGTGT)GATCCGGAAGTCAGTGCGGAGGACCCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605195059_k1tl AATTCTAATTCAAGAAATACAGCTTCTCAGGG(+CAGTGT)GATCCTTTAGGATCTGTGTTCTTATTGCACA NGG 10 8 17 8 24 0 100 5 10
    240605195058_ci4s AGCCATGTGCAATAAGAACACAGATCCTAAAG(+CAGTGT)GATCCCCTGAGAAGCTGTATTTCTTGAATTA NGG 10 8 17 8 24 0 100 5 10
    240605195056_zdsp TTCATTTTAAGCTGGACCCGGTCTCTGCAAAG(+CAGTGT)GTACCAGAATCTCTCGGCCCTGCACCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605195054_2wty TCAGAAAAGGGGTGCAGGGCCGAGAGATTCTG(+CAGTGT)GTACCTTTGCAGAGACCGGGTCCAGCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605195053_m7li TCTTAGGGAATAAAGGGATCTTAAATGCTGAG(+CAGTGT)GATCCAGCATCATGGAATCCAAGTGCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605195052_wsg0 TAAGCCCTTTGCACTTGGATTCCATGATGCTG(+CAGTGT)GATCCTCAGCATTTAAGATCCCTTTATTCCC NGG 10 8 17 8 24 0 100 5 10
    240605195049_45f6 GTGGCTCACAACTGTCTGTAACTCTGTCCAGG(+CAGTGT)GATCCAATGGCCACTTCTGAACTCCCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605195047_b8xy CCCGTGCCTGGGGAGTTCAGAAGTGGCCATTG(+CAGTGT)GATCCCTGGACAGAGTTACAGACAGTTGTGA NGG 10 8 17 8 24 0 100 5 10