Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195138_zxf9 AAAGTCTCTGTGCTCTACTGCCAAGAGGCCGG(+CAGTGT)GATCCTCTGGCCCACTGTTTCCCCAGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240605195136_gb2r CCAGAGTGAGCTGGGGAAACAGTGGGCCAGAG(+CAGTGT)GATCCCGGCCTCTTGGCAGTAGAGCACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605195135_n6mz TCCCAAATACTTCTTCCACTTCCACATTAGAG(+CAGTGT)GATCCACAAATCAAAATATCATCCATACTAC NGG 10 8 17 8 24 0 100 5 10
    240605195133_faov AAAGCGTAGTATGGATGATATTTTGATTTGTG(+CAGTGT)GATCCTCTAATGTGGAAGTGGAAGAAGTATT NGG 10 8 17 8 24 0 100 5 10
    240605195132_lmei ATACTGGGGTGCTTTAGGGGTCCAGAGTACTG(+CAGTGT)GTACCACTGTCCTATGGCCTGGGATGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605195131_ogj1 TGGAACCTGACATCCCAGGCCATAGGACAGTG(+CAGTGT)GTACCAGTACTCTGGACCCCTAAAGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240605195130_x75z AAATACAGACTTCGCTCTCTAGGCTTTTGCTG(+CAGTGT)GTACCAGGAACCCAATATGGCCCTTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605195128_htgf CCTGTAGCCTCAAGGGCCATATTGGGTTCCTG(+CAGTGT)GTACCAGCAAAAGCCTAGAGAGCGAAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605195126_5ze1 CCCAGCCAGTGGAAGCTGCAAGAAGACATATG(+CAGTGT)GTACCACTGGGCTGCTGCTCTCCCAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240605195124_27c7 AGCTCTAAGTCTGGGAGAGCAGCAGCCCAGTG(+CAGTGT)GTACCATATGTCTTCTTGCAGCTTCCACTGG NGG 10 8 17 8 24 0 100 5 10