Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195236_dr65 AGGGATGCACTCTGGGCTCCAGAAGCAGGCAG(+CAGTGT)GTACCCGAGTCTATGCTAGGACCTTAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605195234_p9x8 TTATGGTGCTTAAGGTCCTAGCATAGACTCGG(+CAGTGT)GTACCTGCCTGCTTCTGGAGCCCAGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605195232_0amu CAGAGGAGACCCTACGAACGCCTAAGAGCCAG(+CAGTGT)GATCCTCCCAGCCCCCAGCCCGCCACCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605195230_ic9l TGCTGGAAGGGTGGCGGGCTGGGGGCTGGGAG(+CAGTGT)GATCCTGGCTCTTAGGCGTTCGTAGGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605195228_b9h1 CTATGGCACGTTTCTGTTACCCAATGCCAAGG(+CAGTGT)GTACCATCTTGCCACCTTGTCTCTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605195225_wgpb TCTCTATTCTTGAGAGACAAGGTGGCAAGATG(+CAGTGT)GTACCCTTGGCATTGGGTAACAGAAACGTGC NGG 10 8 17 8 24 0 100 5 10
    240605195224_4g7s CACTCCTCCTAGGGGAAAGCTGAACCCTGTGG(+CAGTGT)GATCCCGGGAACAGGGATAGCCCAGCTCCGG NGG 10 8 17 8 24 0 100 5 10
    240605195221_hgws CAGAACCGGAGCTGGGCTATCCCTGTTCCCGG(+CAGTGT)GATCCCACAGGGTTCAGCTTTCCCCTAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605195220_jz1p GCCACGTGGTGGCTCACAACCATCTGTAACAG(+CAGTGT)GATCCGATGCCTTCTTCTGGTGCATCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605195219_xez5 GCTGTCTTCAGATGCACCAGAAGAAGGCATCG(+CAGTGT)GATCCTGTTACAGATGGTTGTGAGCCACCAC NGG 10 8 17 8 24 0 100 5 10