Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195328_yv31 AGCAGAGCCCTGGCCCTATTAGCAGCACAGTG(+CAGTGT)GTACCCGAGGGTCTCTGTCAACAAGACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605195326_y5wq TCATTTCCTGTCTTGTTGACAGAGACCCTCGG(+CAGTGT)GTACCACTGTGCTGCTAATAGGGCCAGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605195324_v7rz CACACACAGCACCCATGCTAGCACGCACAGAG(+CAGTGT)GATCCCGTTGATCACTCCAGAAGAGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240605195322_35g6 ACCCAGGTCACCTCTTCTGGAGTGATCAACGG(+CAGTGT)GATCCTCTGTGCGTGCTAGCATGGGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605195320_rwd1 AATTAAATGTTGTCCTTATAAGAGTTGCCTTG(+CAGTGT)GTACCAGGAGAGCCAGGGCTATACAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605195318_x6f0 CAGGGTTTCTCTGTATAGCCCTGGCTCTCCTG(+CAGTGT)GTACCAAGGCAACTCTTATAAGGACAACATT NGG 10 8 17 8 24 0 100 5 10
    240605195316_9kx5 TTCAAAACAATGAAATAAAACCTAAGCCGTAG(+CAGTGT)GTACCTAAAATGAGGTTATTTCTCAACCCCT NGG 10 8 17 8 24 0 100 5 10
    240605195315_eute GGATAAGGGGTTGAGAAATAACCTCATTTTAG(+CAGTGT)GTACCTACGGCTTAGGTTTTATTTCATTGTT NGG 10 8 17 8 24 0 100 5 10
    240605195313_x2cf CAAGAGTCAGTGAAAGAGTCCCGAGAGCGCTG(+CAGTGT)GATCCCCATCCTTGTCCAGGGTGAAGTCACT NGG 10 8 17 8 24 0 100 5 10
    240605195311_w73w TCCCAAGTGACTTCACCCTGGACAAGGATGGG(+CAGTGT)GATCCAGCGCTCTCGGGACTCTTTCACTGAC NGG 10 8 17 8 24 0 100 5 10