Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195416_ux0s TGCAGCCATAGCAGGGCAACAGCAAGGCCTGG(+CAGTGT)GATCCCTTTGTCCAGACCCTGGGCAAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605195414_bd1h GCTTCTGGCCTTGCCCAGGGTCTGGACAAAGG(+CAGTGT)GATCCCAGGCCTTGCTGTTGCCCTGCTATGG NGG 10 8 17 8 24 0 100 5 10
    240605195413_qcrw CAACTCCCACAGGCACTTGAGGTGACACAGAG(+CAGTGT)GTACCTGATACTCGTAGGAGTCAGATGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605195412_uotw GGACCTGAGCATCTGACTCCTACGAGTATCAG(+CAGTGT)GTACCTCTGTGTCACCTCAAGTGCCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605195410_2tzz TTTTTCAGTCCCAGGTTGACCTCACAGCAAGG(+CAGTGT)GATCCAGGTCCTGGAGTGCTTAGGCATACAG NGG 10 8 17 8 24 0 100 5 10
    240605195408_nld8 TGATGCTGTATGCCTAAGCACTCCAGGACCTG(+CAGTGT)GATCCCTTGCTGTGAGGTCAACCTGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240605195406_nb6g CCTCATGGGCTCTCCGTGGTGAAGAACAAAGG(+CAGTGT)GTACCCCAATGGCCTCTCACCATCACTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605195404_n2mr CTGGAGGACAGTGATGGTGAGAGGCCATTGGG(+CAGTGT)GTACCCTTTGTTCTTCACCACGGAGAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605195403_e28t CCACTTCTCCTGGAGTCCCAATCAGCACACAG(+CAGTGT)GATCCTCATGTGGGTGTAGGCTCAGCTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605195401_fzsu CACCAGGACAGCTGAGCCTACACCCACATGAG(+CAGTGT)GATCCTGTGTGCTGATTGGGACTCCAGGAGA NGG 10 8 17 8 24 0 100 5 10