Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195532_4pn4 CTGCAGGTGCTGGTCCTGCAAAGTCCAAGCGG(+CAGTGT)GTACCCCTTCCTGGGTGATGACCCTGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605195530_w9la GCATTCTGCACAGGGTCATCACCCAGGAAGGG(+CAGTGT)GTACCCGCTTGGACTTTGCAGGACCAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605195528_386q GTTGTGGGGTTCGTAGCAGTCCCAGGATCCTG(+CAGTGT)GTACCTAAGGCAGCTGGGACCCAGAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605195526_h5rn GGAAACTCTGTTCTGGGTCCCAGCTGCCTTAG(+CAGTGT)GTACCAGGATCCTGGGACTGCTACGAACCCC NGG 10 8 17 8 24 0 100 5 10
    240605195523_zexv ACATAGAGTTGTGGGGTTCGTAGCAGTCCCAG(+CAGTGT)GATCCTGGTACCTAAGGCAGCTGGGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605195522_j1r6 CTGTTCTGGGTCCCAGCTGCCTTAGGTACCAG(+CAGTGT)GATCCTGGGACTGCTACGAACCCCACAACTC NGG 10 8 17 8 24 0 100 5 10
    240605195520_2iac TGAAGATACACCTAATCCTCAAAGAACAGGAG(+CAGTGT)GATCCAGGGAGTGTGCAGGTTTGTTGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605195517_j39u TCCTCCACCCCAACAAACCTGCACACTCCCTG(+CAGTGT)GATCCTCCTGTTCTTTGAGGATTAGGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240605195515_k69s CGGAGCAGTGTCAGAGGCCCAGGTGGATGCTG(+CAGTGT)GTACCAGCAGATTAAACAGCAGCCATCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605195513_m7fl TCCCAGCAGGATGGCTGCTGTTTAATCTGCTG(+CAGTGT)GTACCAGCATCCACCTGGGCCTCTGACACTG NGG 10 8 17 8 24 0 100 5 10