Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195644_idur CAGGGTCTGCAAGTGGGCAAAGGCCTATTCAG(+CAGTGT)GATCCTGGGTGGTGGAGGGGACAGCAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605195642_n41t AGTGACAACCTGCTGTCCCCTCCACCACCCAG(+CAGTGT)GATCCTGAATAGGCCTTTGCCCACTTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605195640_5fft AGTGGGGAAGGTCTCAGGAGAGTCTACAGTAG(+CAGTGT)GATCCTCTACTACTGCAGACTGGATTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605195639_gkzy CTAGAAAAGAAATCCAGTCTGCAGTAGTAGAG(+CAGTGT)GATCCTACTGTAGACTCTCCTGAGACCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605195636_vkf2 GCACACCTCCTCAGCTGAGGGGTCCAACCCTG(+CAGTGT)GTACCGCTGGGATCCAGGGACCGAACCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605195634_59z5 ACCATGTGGGGTTCGGTCCCTGGATCCCAGCG(+CAGTGT)GTACCAGGGTTGGACCCCTCAGCTGAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605195633_zlb5 GGTAGGCTGTGCCTGCTAGACCTCCCCTGAAG(+CAGTGT)GTACCTTGCTGTTGTCCCTGATGCAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605195631_nki3 TGCCATCAGCCTGCATCAGGGACAACAGCAAG(+CAGTGT)GTACCTTCAGGGGAGGTCTAGCAGGCACAGC NGG 10 8 17 8 24 0 100 5 10
    240605195630_twta GAGGGCAGGATTGGGCATGGGGATGCTGTAAG(+CAGTGT)GATCCAGCTTGTTTTGGCCACTTGGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240605195629_ndp1 TGAGACCCATCCCAAGTGGCCAAAACAAGCTG(+CAGTGT)GATCCTTACAGCATCCCCATGCCCAATCCTG NGG 10 8 17 8 24 0 100 5 10