Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195704_7ciq TCCCTGCCCTCCCCTGTCCCGGCCCAGCCTGG(+CAGTGT)GATCCATGGGAGTGGGAGGGGTAACTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605195701_3uwx GCCTGCCTCAAGTTACCCCTCCCACTCCCATG(+CAGTGT)GATCCCAGGCTGGGCCGGGACAGGGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605195700_urio CTGCATCCCAAGTGCTTTGTTAAAGATGTAAG(+CAGTGT)GTACCAAACCCAAGTCTATTCAGAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240605195658_p92m ACCAAAAATATTTCTGAATAGACTTGGGTTTG(+CAGTGT)GTACCTTACATCTTTAACAAAGCACTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605195655_i9go CCCTCCTGGGAACAGTATCTGGGCTAACCTGG(+CAGTGT)GATCCAGTAATGAATTCACTTGTGGCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605195654_dcuv AAGGGTGGGAGCCACAAGTGAATTCATTACTG(+CAGTGT)GATCCCAGGTTAGCCCAGATACTGTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605195651_cixb CTCCAGCCACTCTGCTGTCTTCCAGGGTTGGG(+CAGTGT)GATCCCGCAGGGTCAGCACCCCACACCTCTC NGG 10 8 17 8 24 0 100 5 10
    240605195649_62hp GCTGGGAGAGGTGTGGGGTGCTGACCCTGCGG(+CAGTGT)GATCCCCAACCCTGGAAGACAGCAGAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240605195646_fkvf GCACCCAGCTGGCCTGATGTGGCTCTCCGGGG(+CAGTGT)GTACCACAGAACACCCCTGTACATACCACAG NGG 10 8 17 8 24 0 100 5 10
    240605195645_28eq CAGGGCTGTGGTATGTACAGGGGTGTTCTGTG(+CAGTGT)GTACCCCCGGAGAGCCACATCAGGCCAGCTG NGG 10 8 17 8 24 0 100 5 10