Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195805_vq0y GGGTAAAATAAGTAAGTTTAAAGGGAATTAAG(+CAGTGT)GATCCAGATAATACAGAAATGCCTCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240605195803_y5rv TCAGACCAAAGGAGGCATTTCTGTATTATCTG(+CAGTGT)GATCCTTAATTCCCTTTAAACTTACTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605195801_lyc5 ATCATCTGATGTGGGTGCTAGAAAACATCTGG(+CAGTGT)GTACCCAACATGATCAGTACTATTGAGACAC NGG 10 8 17 8 24 0 100 5 10
    240605195759_61ea AGAATGTGTCTCAATAGTACTGATCATGTTGG(+CAGTGT)GTACCCAGATGTTTTCTAGCACCCACATCAG NGG 10 8 17 8 24 0 100 5 10
    240605195756_l4ff TCCCACACATGTGGAAGAGGCCGTGTCCCAGG(+CAGTGT)GATCCACGTGCCTCTCTACCAATCACACCCG NGG 10 8 17 8 24 0 100 5 10
    240605195754_7yhc ACGAGCGGGTGTGATTGGTAGAGAGGCACGTG(+CAGTGT)GATCCCTGGGACACGGCCTCTTCCACATGTG NGG 10 8 17 8 24 0 100 5 10
    240605195752_oi8b TGCGGACCATCTGCTCTGGGCTCGGGAGGGAG(+CAGTGT)GTACCATGTCAGGAGCAAATGTCCCCAGACT NGG 10 8 17 8 24 0 100 5 10
    240605195750_0jcz CCATCAGTCTGGGGACATTTGCTCCTGACATG(+CAGTGT)GTACCTCCCTCCCGAGCCCAGAGCAGATGGT NGG 10 8 17 8 24 0 100 5 10
    240605195748_ot38 CCATCACCCACAGACCAGGCAATCCTGGCCTG(+CAGTGT)GTACCTGCGCCCAGCACCAACAGATGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605195747_c7st CCATTCTGCCCATCTGTTGGTGCTGGGCGCAG(+CAGTGT)GTACCAGGCCAGGATTGCCTGGTCTGTGGGT NGG 10 8 17 8 24 0 100 5 10