Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195724_h6zl GTTCCTGTAATAGAAGTTATTAGTGCAGGCGG(+CAGTGT)GATCCGGCACAGCCAGCTCCTGGCATCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605195723_lwy6 TGAGGGCAGGATGCCAGGAGCTGGCTGTGCCG(+CAGTGT)GATCCCGCCTGCACTAATAACTTCTATTACA NGG 10 8 17 8 24 0 100 5 10
    240605195721_r4jl AGATACCTCCGTTTTCTTTGTGACCGGCGTTG(+CAGTGT)GATCCTGGATAGTTATGGCTGGCTGCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605195719_s47h CGCAGTGACTGCAGCCAGCCATAACTATCCAG(+CAGTGT)GATCCAACGCCGGTCACAAAGAAAACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605195717_0m6s CACCAGCCCAGCTCCTGTCCACCGCTGCTCGG(+CAGTGT)GTACCCAGGGCTACCCCAGACCCTGCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605195714_uvcd GAGCCTCTCTGCAGGGTCTGGGGTAGCCCTGG(+CAGTGT)GTACCCGAGCAGCGGTGGACAGGAGCTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605195713_a6kx GGCCTCAGTGCTCAGCCTGCTCTTTAATAAAG(+CAGTGT)GTACCTCTTTTCTGAGCCATGTCGGAATGAG NGG 10 8 17 8 24 0 100 5 10
    240605195712_wsjh CTCCACTCATTCCGACATGGCTCAGAAAAGAG(+CAGTGT)GTACCTTTATTAAAGAGCAGGCTGAGCACTG NGG 10 8 17 8 24 0 100 5 10
    240605195710_9gds CCTTACCCTGTACTAGGCCCATCAGTCACAGG(+CAGTGT)GTACCTGGGGCCACTATGCACCATGGTCAGT NGG 10 8 17 8 24 0 100 5 10
    240605195706_b0yp AGTAAACTGACCATGGTGCATAGTGGCCCCAG(+CAGTGT)GTACCCTGTGACTGATGGGCCTAGTACAGGG NGG 10 8 17 8 24 0 100 5 10