Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195745_g8ra CACAGCACCCAGCCTCACTACCCCAGCATGGG(+CAGTGT)GTACCCAGCTGGATACTCCCAAAATGTCACA NGG 10 8 17 8 24 0 100 5 10
    240605195743_w4of TGGGATGTGACATTTTGGGAGTATCCAGCTGG(+CAGTGT)GTACCCCATGCTGGGGTAGTGAGGCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240605195740_ze1w ATTCTAGCCCACACCTGGGACCACCACCCCAG(+CAGTGT)GTACCTGGGGGACAGTGAGGCAAGACACAGA NGG 10 8 17 8 24 0 100 5 10
    240605195737_g1ya GGACCTCTGTGTCTTGCCTCACTGTCCCCCAG(+CAGTGT)GTACCTGGGGTGGTGGTCCCAGGTGTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240605195734_m7zk GGGCTGTTCCATTTCTTTACTGAGACACCCAG(+CAGTGT)GATCCATTTGGCAAGTGACCCAGAGCAACAG NGG 10 8 17 8 24 0 100 5 10
    240605195733_olu8 GGACACTGTTGCTCTGGGTCACTTGCCAAATG(+CAGTGT)GATCCTGGGTGTCTCAGTAAAGAAATGGAAC NGG 10 8 17 8 24 0 100 5 10
    240605195732_xkla CTGTTGTGTTTTTTTTTTCCTTTTTGTTATTG(+CAGTGT)GTACCAGGCAATTAAAAAAAAAAAAAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605195730_0yj9 CCTCTACTTTTTTTTTTTTTTTTAATTGCCTG(+CAGTGT)GTACCAATAACAAAAAGGAAAAAAAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605195727_3b0o CCCCTGGGCCCATTCTGCTGGCCCCAAGGGGG(+CAGTGT)GATCCTGAGACATCTGGGGGGGGGGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605195726_van4 GCGAGCCCTCCCCCCCCCCCCAGATGTCTCAG(+CAGTGT)GATCCCCCCTTGGGGCCAGCAGAATGGGCCC NGG 10 8 17 8 24 0 100 5 10