Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195549_qzoi AAGATGGCAGGCTGTCTAGGCACCTGGCCAGG(+CAGTGT)GATCCAAAGGACATCAGGCTTGGGGTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605195547_426n ACCCTAAAGGACCCCAAGCCTGATGTCCTTTG(+CAGTGT)GATCCCTGGCCAGGTGCCTAGACAGCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605195545_k0tk GGTGTCTCACCTGCCCCACGCCAAGCCCTGAG(+CAGTGT)GTACCAGGTGGACAGTAACGACCCCAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605195544_buyd GGGTCAGAGCTGGGGTCGTTACTGTCCACCTG(+CAGTGT)GTACCTCAGGGCTTGGCGTGGGGCAGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605195542_43vv GCCTAGTTTGGCTCCTATGGAAGAGACAGCAG(+CAGTGT)GATCCTTCCCGGTTGACTACTGTCACATTCA NGG 10 8 17 8 24 0 100 5 10
    240605195541_op7r ACACCTGAATGTGACAGTAGTCAACCGGGAAG(+CAGTGT)GATCCTGCTGTCTCTTCCATAGGAGCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605195539_wvqk GCTGAGGGATGGCTCAGGGGTTAAGAGAACTG(+CAGTGT)GATCCTCTGCCACAGAGCCTTAGTTCCATTC NGG 10 8 17 8 24 0 100 5 10
    240605195538_vwn4 ACTGGGAATGGAACTAAGGCTCTGTGGCAGAG(+CAGTGT)GATCCAGTTCTCTTAACCCCTGAGCCATCCC NGG 10 8 17 8 24 0 100 5 10
    240605195536_jifa CCCTGCTTTGAGCTCTGCTACCTATGGGAGGG(+CAGTGT)GATCCACAGGTGCCCATCCTCCTCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605195534_lt73 TGAAGTTCTAGGGAGGAGGATGGGCACCTGTG(+CAGTGT)GATCCCCTCCCATAGGTAGCAGAGCTCAAAG NGG 10 8 17 8 24 0 100 5 10