Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195310_8jfe TCTGTCCTTAGACACACCAGAAAAGGGCATCG(+CAGTGT)GATCCCATTACAGATAGCTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605195308_7ag8 ACCACATGGTGGCTCACAGCTATCTGTAATGG(+CAGTGT)GATCCGATGCCCTTTTCTGGTGTGTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605195307_1h2f CAGCCTCGAGAAATTTCTCCTATTATATTTGG(+CAGTGT)GATCCAGCATGTCCCAAGACAGGGACGACTC NGG 10 8 17 8 24 0 100 5 10
    240605195305_cvve TTATTGAGTCGTCCCTGTCTTGGGACATGCTG(+CAGTGT)GATCCCAAATATAATAGGAGAAATTTCTCGA NGG 10 8 17 8 24 0 100 5 10
    240605195304_ud1w TTCTGGGGGCAGAGTTCTGGTTACTTTTCACG(+CAGTGT)GATCCGCAGAAGAACTGCCTAGGTGCCTAGG NGG 10 8 17 8 24 0 100 5 10
    240605195302_dlh8 ACACCCCTAGGCACCTAGGCAGTTCTTCTGCG(+CAGTGT)GATCCGTGAAAAGTAACCAGAACTCTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605195300_o9i4 GGAACCAGGGATTCTGTAGCAGACATGGGCTG(+CAGTGT)GATCCATACACGGTCCCTGTGAGTTCATTTC NGG 10 8 17 8 24 0 100 5 10
    240605195259_b416 ATTGAGAAATGAACTCACAGGGACCGTGTATG(+CAGTGT)GATCCAGCCCATGTCTGCTACAGAATCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605195254_22i9 AACTGAATGGCTAATTTATTGAGAACCTATTG(+CAGTGT)GATCCACTCACAGGATTGAAGGACGCCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605195253_bbtu CTCATTGAGGGCGTCCTTCAATCCTGTGAGTG(+CAGTGT)GATCCAATAGGTTCTCAATAAATTAGCCATT NGG 10 8 17 8 24 0 100 5 10