Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195251_1g5x CACACCCACCTCCATCCCGAGTGCCTGGATGG(+CAGTGT)GTACCGAGCTTAATGGACACCTTTGAAATAG NGG 10 8 17 8 24 0 100 5 10
    240605195250_7yeo CAGGTCTATTTCAAAGGTGTCCATTAAGCTCG(+CAGTGT)GTACCCATCCAGGCACTCGGGATGGAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605195248_80vd TATTCTTCTAAAGGGCTGTGAAATGCCCTAAG(+CAGTGT)GTACCCATACCTCAATTGAAATGGCATTCTC NGG 10 8 17 8 24 0 100 5 10
    240605195246_rrmg AGCCAGAGAATGCCATTTCAATTGAGGTATGG(+CAGTGT)GTACCTTAGGGCATTTCACAGCCCTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605195245_2yy4 TGATAATGCACACTTTCTCTAAAGGGGAGCTG(+CAGTGT)GATCCCACAGTTGGACAGTTTTCACTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605195243_iqb1 GTACATTTTAAGTGAAAACTGTCCAACTGTGG(+CAGTGT)GATCCAGCTCCCCTTTAGAGAAAGTGTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605195241_kg6d CCCATCCTGCACAGGGAGCTGGATGCTCTTGG(+CAGTGT)GATCCATACACATGTGGATCTGAGTTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605195240_curf ACAGAACACTAACTCAGATCCACATGTGTATG(+CAGTGT)GATCCCAAGAGCATCCAGCTCCCTGTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605195239_1yg3 CCTTAATAATGGCCCTGATGTACTGCAAGTAG(+CAGTGT)GATCCTGTCTCCAACCCCAGCTCAGACAGTA NGG 10 8 17 8 24 0 100 5 10
    240605195237_zij3 GGCTGTACTGTCTGAGCTGGGGTTGGAGACAG(+CAGTGT)GATCCTACTTGCAGTACATCAGGGCCATTAT NGG 10 8 17 8 24 0 100 5 10