Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195217_jd7t GCCCCCAATGTCTGCTGTGCGCAGCAGCAAGG(+CAGTGT)GTACCGATGAGAAGCCTAGTTAAGCCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605195215_skpq CCTCTCACAGGGCTTAACTAGGCTTCTCATCG(+CAGTGT)GTACCCTTGCTGCTGCGCACAGCAGACATTG NGG 10 8 17 8 24 0 100 5 10
    240605195213_yqbv GAAGCCTAGTTAAGCCCTGTGAGAGGGCTAGG(+CAGTGT)GATCCAAGTAGTGAAAGCCAGAAGAGGCGAG NGG 10 8 17 8 24 0 100 5 10
    240605195212_h90v AGCATCTCGCCTCTTCTGGCTTTCACTACTTG(+CAGTGT)GATCCCTAGCCCTCTCACAGGGCTTAACTAG NGG 10 8 17 8 24 0 100 5 10
    240605195210_4t3c CAGGGGCTATCAGGCTGAGAGATGCCAAACCG(+CAGTGT)GTACCCGCCAAAGGACTAGAACCCCCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605195208_yxfo TGTTCCTTCTGGGGGTTCTAGTCCTTTGGCGG(+CAGTGT)GTACCGGTTTGGCATCTCTCAGCCTGATAGC NGG 10 8 17 8 24 0 100 5 10
    240605195206_575x GAGGCTCTGTCCTCTCCAGGCTCTTGTCTCGG(+CAGTGT)GTACCACCCTCAACAGGCAGTCACACAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605195204_saxl GGGCTCTGCTGTGTGACTGCCTGTTGAGGGTG(+CAGTGT)GTACCCGAGACAAGAGCCTGGAGAGGACAGA NGG 10 8 17 8 24 0 100 5 10
    240605195203_cf8w ACTGTCTTCAGACACACCAGAAGAAGGCATCG(+CAGTGT)GATCCCCCATTACAGATGGTTGTGAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240605195200_a1pb CACATGGTGGCTCACAACCATCTGTAATGGGG(+CAGTGT)GATCCGATGCCTTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10