Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605195345_ixpf TAGAAAACTTGCCTAGAATGTACAAGGCCTTG(+CAGTGT)GATCCAAATCCCAGAACTGCAACTCTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605195343_958t TCTTCTGGGGAGAGTTGCAGTTCTGGGATTTG(+CAGTGT)GATCCAAGGCCTTGTACATTCTAGGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240605195342_w6ec GACATTGCGAGCGTCAGTGGCCGCTGGTAGCG(+CAGTGT)GTACCTTCTGGACACGCACCTGCTATCTTGC NGG 10 8 17 8 24 0 100 5 10
    240605195340_qp08 CCTTTGCAAGATAGCAGGTGCGTGTCCAGAAG(+CAGTGT)GTACCGCTACCAGCGGCCACTGACGCTCGCA NGG 10 8 17 8 24 0 100 5 10
    240605195339_yz6u ATATTACATATGCACTGATTTTTTTTTTCATG(+CAGTGT)GTACCTAAAATGAAGGAGAATTTTGACATCT NGG 10 8 17 8 24 0 100 5 10
    240605195338_if15 ATCCCAGATGTCAAAATTCTCCTTCATTTTAG(+CAGTGT)GTACCATGAAAAAAAAAATCAGTGCATATGT NGG 10 8 17 8 24 0 100 5 10
    240605195336_fotg CAAAGTGCCTTTGTCTGCCTGGAGTCAACTTG(+CAGTGT)GATCCATTGGAAACATAGAAAGGGTCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605195334_04sn CTGTCCCCTGGACCCTTTCTATGTTTCCAATG(+CAGTGT)GATCCAAGTTGACTCCAGGCAGACAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605195332_l8xf CAGGTGGCTGTGGGGGACAGAAGAGGGTGCCG(+CAGTGT)GATCCCCTGGAGCTGGAGTTAGGCTTTAGTT NGG 10 8 17 8 24 0 100 5 10
    240605195330_xz9k TTAGTAACTAAAGCCTAACTCCAGCTCCAGGG(+CAGTGT)GATCCGGCACCCTCTTCTGTCCCCCACAGCC NGG 10 8 17 8 24 0 100 5 10