Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194945_l4qu GCTGTTTTCAAACACACCAGAAGAGGGCATAG(+CAGTGT)GATCCCATTACAGAGGGTTGTGAGTCACGGT NGG 10 8 17 8 24 0 100 5 10
    240605194943_efpz ACCACACCGTGACTCACAACCCTCTGTAATGG(+CAGTGT)GATCCTATGCCCTCTTCTGGTGTGTTTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605194941_fdcv AAAGCAGTGGTTTGTACACACAGTACTGAAAG(+CAGTGT)GTACCCGAGGAGTCGCTACTCACAGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605194939_ogi4 ACATATTTAAACTGTGAGTAGCGACTCCTCGG(+CAGTGT)GTACCTTTCAGTACTGTGTGTACAAACCACT NGG 10 8 17 8 24 0 100 5 10
    240605194937_vk65 CTGACAGACAGCCAAAATGGGGCAGGTTCCAG(+CAGTGT)GATCCTGATTGGTCGAGTGCTGGAGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605194935_ckct GGCTCTGCCTCCTCCAGCACTCGACCAATCAG(+CAGTGT)GATCCTGGAACCTGCCCCATTTTGGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605194934_m64y AGCGTTTGCCAAGATGGAGCTTTATACAGATG(+CAGTGT)GTACCTCCTAAGTCCCCTCTCTGGCCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605194932_pvbr ATGCAATGCTGGCCAGAGAGGGGACTTAGGAG(+CAGTGT)GTACCATCTGTATAAAGCTCCATCTTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240605194930_t0l8 GCATATATGTATGTAAGCAGACCACGTGGGTG(+CAGTGT)GTACCTGTAGGGTATCAGAAGAGGGCATCAG NGG 10 8 17 8 24 0 100 5 10
    240605194928_tri6 GGGATCTGATGCCCTCTTCTGATACCCTACAG(+CAGTGT)GTACCACCCACGTGGTCTGCTTACATACATA NGG 10 8 17 8 24 0 100 5 10