Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194835_q8at TTTTAATATTTAATGAGCAACAAACACACTGG(+CAGTGT)GATCCAGGACTAAAGAAATAATACCATTCAT NGG 10 8 17 8 24 0 100 5 10
    240605194833_7fcy ATAGGATGAATGGTATTATTTCTTTAGTCCTG(+CAGTGT)GATCCCAGTGTGTTTGTTGCTCATTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240605194831_j4oq TTCTAGCTGCTAATAGCTTTCTGCTTTTGGTG(+CAGTGT)GTACCTCAGTGAGGTGGCAGTAAATGGATAC NGG 10 8 17 8 24 0 100 5 10
    240605194830_eniu AGAAAGTATCCATTTACTGCCACCTCACTGAG(+CAGTGT)GTACCACCAAAAGCAGAAAGCTATTAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605194828_ky1p GTCTGCTGCCAATGATTTCAACCAGACGGCTG(+CAGTGT)GTACCACCCCAGCTTGGAACTATACCCATCT NGG 10 8 17 8 24 0 100 5 10
    240605194826_ans2 CAAGGAGATGGGTATAGTTCCAAGCTGGGGTG(+CAGTGT)GTACCAGCCGTCTGGTTGAAATCATTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605194824_6e1s TAGAGGGAAGTAGCCAGTACAGAAAGTGCGGG(+CAGTGT)GTACCAAAGTGTGGACAAGAAGTGTTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605194823_dmzi ACCTGAGTTTAACACTTCTTGTCCACACTTTG(+CAGTGT)GTACCCCGCACTTTCTGTACTGGCTACTTCC NGG 10 8 17 8 24 0 100 5 10
    240605194820_wz1s ACACACCAGTTGCTCCGGGAGGACTTCGGTGG(+CAGTGT)GATCCGACGTTAATGGCAGTTCCTCCGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605194819_kphq TGGTGCTTTCGGAGGAACTGCCATTAACGTCG(+CAGTGT)GATCCCACCGAAGTCCTCCCGGAGCAACTGG NGG 10 8 17 8 24 0 100 5 10