Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194817_kjeu CTACCAGAGCAGTGAGACATAAGTGCGCTGAG(+CAGTGT)GTACCCCCCCACACACACACACTTATATAAA NGG 10 8 17 8 24 0 100 5 10
    240605194815_xx6i TTTCATTTATATAAGTGTGTGTGTGTGGGGGG(+CAGTGT)GTACCTCAGCGCACTTATGTCTCACTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240605194812_q1oc TAAAACATGTACTATTTAAAATAAAAAATAGG(+CAGTGT)GTACCACATATTCTGTATCCATTATTTATAC NGG 10 8 17 8 24 0 100 5 10
    240605194811_76v2 CCATTGTATAAATAATGGATACAGAATATGTG(+CAGTGT)GTACCCTATTTTTTATTTTAAATAGTACATG NGG 10 8 17 8 24 0 100 5 10
    240605194809_e4go GAATACATCATCAAATGATAAAAAAAAATAGG(+CAGTGT)GTACCGATCTAAAAGAGAATTCTCAAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605194808_zns4 TTTTCCTCTTTTGAGAATTCTCTTTTAGATCG(+CAGTGT)GTACCCTATTTTTTTTTATCATTTGATGATG NGG 10 8 17 8 24 0 100 5 10
    240605194806_0h25 CACACCTCACAGAGCATGGAGAAGTCAAACTG(+CAGTGT)GTACCTACCAGAGCCTTCACCTCTCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240605194805_q4pv AGGTAGTCCATGAGAGGTGAAGGCTCTGGTAG(+CAGTGT)GTACCAGTTTGACTTCTCCATGCTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605194803_gvir TAGAGTATTTGTAACTGTATTCTCTAGCTGGG(+CAGTGT)GATCCATTGTTTTTATATTTCAAGTACACTG NGG 10 8 17 8 24 0 100 5 10
    240605194802_979w AAATACAGTGTACTTGAAATATAAAAACAATG(+CAGTGT)GATCCCCAGCTAGAGAATACAGTTACAAATA NGG 10 8 17 8 24 0 100 5 10