Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194034_tiqf GCCTGAGATTCCATTTCTTCTTCTCTTCACAG(+CAGTGT)GTACCTGAATTCAGGTGTGTCTCTTGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605194033_w4z0 AATCTCAGGGCAAGAGACACACCTGAATTCAG(+CAGTGT)GTACCTGTGAAGAGAAGAAGAAATGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240605194031_xwbg GCAGAAAGCCTATGTCCTGCATGCTGGAACAG(+CAGTGT)GTACCTGTCTGTGTGTGGCCTTTCCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605194029_0tj4 CCCAGATGTGGGGAAAGGCCACACACAGACAG(+CAGTGT)GTACCTGTTCCAGCATGCAGGACATAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605194026_mf23 CCCATTTCCGTTCACAGTATGTTGGGGCCCAG(+CAGTGT)GTACCAACAGTGTAGGGCTTTCTGATACCCT NGG 10 8 17 8 24 0 100 5 10
    240605194025_ee9q TCCTGAGGGTATCAGAAAGCCCTACACTGTTG(+CAGTGT)GTACCTGGGCCCCAACATACTGTGAACGGAA NGG 10 8 17 8 24 0 100 5 10
    240605194023_qtfa GGGGAGGGAGATGAGGCTGCCCCAAGGCTCTG(+CAGTGT)GTACCTGGCTTTAGTTTTCTGTCTTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605194021_5mac CCTGAGGAAAGAAGACAGAAAACTAAAGCCAG(+CAGTGT)GTACCAGAGCCTTGGGGCAGCCTCATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605194020_pdz4 GAGTATTATGGAGCCCGTGGCTTTGTGCTATG(+CAGTGT)GATCCTGGGATGGGGGCCTCTTACCCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605194017_ztg0 GGCTAGTGGGGGGTAAGAGGCCCCCATCCCAG(+CAGTGT)GATCCATAGCACAAAGCCACGGGCTCCATAA NGG 10 8 17 8 24 0 100 5 10