Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194147_ohu0 AGGATGTTGGTGACCAGTCCCGAGGTCCCCAG(+CAGTGT)GATCCGGGAAGCAGCAGGGTCCTGAGCACTA NGG 10 8 17 8 24 0 100 5 10
    240605194145_d1vq TTGTGTAGTGCTCAGGACCCTGCTGCTTCCCG(+CAGTGT)GATCCTGGGGACCTCGGGACTGGTCACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605194143_ni8g AGAGAATGTGGGGACTGCCAGGGGGGCCAGTG(+CAGTGT)GATCCCTGGCCTTCTTGTCTGTACTAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605194141_itd5 ACACAAGCCTTAGTACAGACAAGAAGGCCAGG(+CAGTGT)GATCCACTGGCCCCCCTGGCAGTCCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240605194139_skgi GTAGCTCTCAAGACGTTCCCTGCACCTGACTG(+CAGTGT)GTACCTTCCTGAGCCTCAGCTTTATCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605194137_e0ib ACTAGGTAAAGATAAAGCTGAGGCTCAGGAAG(+CAGTGT)GTACCAGTCAGGTGCAGGGAACGTCTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605194135_fjvj CCTCATGGGATATCCTAAAGAAGGCAGCCTGG(+CAGTGT)GATCCTACCAGAAACTGATTGCAGCAGACCT NGG 10 8 17 8 24 0 100 5 10
    240605194133_4x7q TGTCCAGGTCTGCTGCAATCAGTTTCTGGTAG(+CAGTGT)GATCCCAGGCTGCCTTCTTTAGGATATCCCA NGG 10 8 17 8 24 0 100 5 10
    240605194131_v8m3 AAGGAAAGGCAGCAGTATGCAAAGTGGAGAAG(+CAGTGT)GTACCTAAGTCCATACTGCCCACAGTAATAG NGG 10 8 17 8 24 0 100 5 10
    240605194130_mugu AAGAACTATTACTGTGGGCAGTATGGACTTAG(+CAGTGT)GTACCTTCTCCACTTTGCATACTGCTGCCTT NGG 10 8 17 8 24 0 100 5 10