Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194111_xfmu CTCTGGGGTCAAATCAATTCCTCTCAAGTGTG(+CAGTGT)GTACCCTAGAGAGTGGTGGCCATCTACATTT NGG 10 8 17 8 24 0 100 5 10
    240605194109_y43h GGAGAAAATGTAGATGGCCACCACTCTCTAGG(+CAGTGT)GTACCACACTTGAGAGGAATTGATTTGACCC NGG 10 8 17 8 24 0 100 5 10
    240605194106_nbpx GGCTCCGATTCCTTCACATTCCAGCTGGCCCG(+CAGTGT)GATCCCTGTGTGGCCATAAGGCCTTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240605194105_yd0y GTGACGAGGGCAAGGCCTTATGGCCACACAGG(+CAGTGT)GATCCGGGCCAGCTGGAATGTGAAGGAATCG NGG 10 8 17 8 24 0 100 5 10
    240605194102_vqbs GGGGCACATCTGTGGAGAGGCAGAAGCCAAGG(+CAGTGT)GATCCTGGCTGTGCCCTATGATTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240605194100_0jfl GTCAAGATCTGGGAATCATAGGGCACAGCCAG(+CAGTGT)GATCCCTTGGCTTCTGCCTCTCCACAGATGT NGG 10 8 17 8 24 0 100 5 10
    240605194057_6sef TTTTTACAAGGCTTATAAATCCTTTAGTGTGG(+CAGTGT)GATCCTGTCCCACCATCGGCAGCTGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605194056_iqz8 TGTCCCAACCACAGCTGCCGATGGTGGGACAG(+CAGTGT)GATCCCACACTAAAGGATTTATAAGCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240605194055_onq0 ACCAATGGCACCAGGGTCAGAAGACTGACTTG(+CAGTGT)GATCCGTCACAGAACCCTTTTCAGAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605194053_b52k GAGATGCAGCCTCTGAAAAGGGTTCTGTGACG(+CAGTGT)GATCCAAGTCAGTCTTCTGACCCTGGTGCCA NGG 10 8 17 8 24 0 100 5 10