Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194223_d7vf ACTAACTTGAGGTGAGGCAGCAAAAGGGCCTG(+CAGTGT)GTACCACCAAATGGTGGCTTCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240605194221_4i16 TGACCCCACGGGGTGGAAGCCACCATTTGGTG(+CAGTGT)GTACCAGGCCCTTTTGCTGCCTCACCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240605194220_ndm5 AGACCAGCCTTCTCAGGTTCTCTGGCTCGTGG(+CAGTGT)GATCCTTCCAGGGAGCTACCTACAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605194218_e78k TAACCCAAGCTCTGTAGGTAGCTCCCTGGAAG(+CAGTGT)GATCCCACGAGCCAGAGAACCTGAGAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240605194215_rzc7 CCAAGGAATGGAAGGCCCAGGTCTTTCTCCAG(+CAGTGT)GTACCGTACAGCTGGCCCCTTTGCTTACTGC NGG 10 8 17 8 24 0 100 5 10
    240605194214_4mvx GCAGAGCAGTAAGCAAAGGGGCCAGCTGTACG(+CAGTGT)GTACCTGGAGAAAGACCTGGGCCTTCCATTC NGG 10 8 17 8 24 0 100 5 10
    240605194213_ts66 TCAATGGTGCTTTAAGGACGTGTGCAGGATAG(+CAGTGT)GTACCTTAGAGGAGCAGCCTAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605194211_19tv CATCAGCAGAGAAACTAGGCTGCTCCTCTAAG(+CAGTGT)GTACCTATCCTGCACACGTCCTTAAAGCACC NGG 10 8 17 8 24 0 100 5 10
    240605194210_ls59 GTCCTGTTAGATGGAATCCTGTCATCTCATTG(+CAGTGT)GTACCCAGGGAGCCCAACTTCTTAGTAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605194207_z4g1 CAAGAGAGCTACTAAGAAGTTGGGCTCCCTGG(+CAGTGT)GTACCAATGAGATGACAGGATTCCATCTAAC NGG 10 8 17 8 24 0 100 5 10