Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194327_ml82 TGACTAGTGTACCTGCTGCAGATGTGTCTTTG(+CAGTGT)GATCCCCGGTTTTTACAGGTTCATGGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605194324_5xmu TAGAACTGCCCCATGAACCTGTAAAAACCGGG(+CAGTGT)GATCCAAAGACACATCTGCAGCAGGTACACT NGG 10 8 17 8 24 0 100 5 10
    240605194323_fnx5 CAGCAGGGTTGGGGGCACAATTGTATGAGAAG(+CAGTGT)GATCCATGCCTTGCAGCCTGCTTTGGCCACA NGG 10 8 17 8 24 0 100 5 10
    240605194322_clsc TGTTTTGTGGCCAAAGCAGGCTGCAAGGCATG(+CAGTGT)GATCCTTCTCATACAATTGTGCCCCCAACCC NGG 10 8 17 8 24 0 100 5 10
    240605194320_c9dh CCAGTCTGGAAATGAAAGCTGCTTCCAGTCAG(+CAGTGT)GATCCATTGTAAGAAAAGTGCGTTCGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605194319_vy2y TCCATGCTCACGAACGCACTTTTCTTACAATG(+CAGTGT)GATCCTGACTGGAAGCAGCTTTCATTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605194317_vcx8 GCCCACTGGAGGAATCCGGGGCTTGGCAGTGG(+CAGTGT)GATCCTGCTGAACTGTACTTCGAATACATCC NGG 10 8 17 8 24 0 100 5 10
    240605194316_94m3 AATTTGGATGTATTCGAAGTACAGTTCAGCAG(+CAGTGT)GATCCCACTGCCAAGCCCCGGATTCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605194315_h7ec TCCCTCCGTTCTGGGCTGTTAGCACCACTCAG(+CAGTGT)GTACCAAGGACTGCTCTGAGCACAGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240605194313_n9ks AAGTCTCTCTACTGTGCTCAGAGCAGTCCTTG(+CAGTGT)GTACCTGAGTGGTGCTAACAGCCCAGAACGG NGG 10 8 17 8 24 0 100 5 10