Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194312_a6fa AGCCAACAGGAAAAACTACTGAAAATGTCATG(+CAGTGT)GATCCCATGTCAGTCTGGCACTTGATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605194310_xlge GGCACAAAGAATCAAGTGCCAGACTGACATGG(+CAGTGT)GATCCATGACATTTTCAGTAGTTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605194308_d4a5 CACTAACCACAACACAGTACAAGTGTCTCTGG(+CAGTGT)GTACCCCGAACATCTCCCCGAAGAGTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605194306_xwbq GAGGACTAGAACTCTTCGGGGAGATGTTCGGG(+CAGTGT)GTACCCAGAGACACTTGTACTGTGTTGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605194305_4nl2 ATATTCATGTTTATTCGCTCTCTACCAAGTAG(+CAGTGT)GATCCATTTTTGATAAAGCAGTCTTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605194304_ojle GAAGAAACCCAAAGACTGCTTTATCAAAAATG(+CAGTGT)GATCCTACTTGGTAGAGAGCGAATAAACATG NGG 10 8 17 8 24 0 100 5 10
    240605194302_o0gt GTGTGGTGCAACCTAGAGCCCATCAGAGGTGG(+CAGTGT)GTACCTATGTATACAAGTCCCACCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605194301_ghz1 CTGACAAAGCCAGGTGGGACTTGTATACATAG(+CAGTGT)GTACCCACCTCTGATGGGCTCTAGGTTGCAC NGG 10 8 17 8 24 0 100 5 10
    240605194259_pgwj ACTGGAAGCCCAAGCCAAACGCCCAAGCCTCG(+CAGTGT)GTACCTATTAGGACTTACACTGACCTCGCAC NGG 10 8 17 8 24 0 100 5 10
    240605194257_9yzi TCCGTGTGCGAGGTCAGTGTAAGTCCTAATAG(+CAGTGT)GTACCGAGGCTTGGGCGTTTGGCTTGGGCTT NGG 10 8 17 8 24 0 100 5 10