Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194404_tk1z GTGTGCTGGTCCAGCAGACAGCAGGACCAGAG(+CAGTGT)GTACCCGGCGACTCTTGGCAAACACCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605194402_ek0k GCCCAAGAAAGGTGTTTGCCAAGAGTCGCCGG(+CAGTGT)GTACCTCTGGTCCTGCTGTCTGCTGGACCAG NGG 10 8 17 8 24 0 100 5 10
    240605194400_5tf3 AGCAGAGTTTTAAAACCATCTCAGCCACCTCG(+CAGTGT)GATCCCAGAGAAGGACGACACTGAGTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605194358_2h0r GGGGCTCAAAACTCAGTGTCGTCCTTCTCTGG(+CAGTGT)GATCCGAGGTGGCTGAGATGGTTTTAAAACT NGG 10 8 17 8 24 0 100 5 10
    240605194357_kp00 CTGAAGTCTAGAAGCGTTGTCTCTCATCTGTG(+CAGTGT)GTACCCTCCCTGCGGTTCTCCAGTACCACTC NGG 10 8 17 8 24 0 100 5 10
    240605194355_d05x AAACTGAGTGGTACTGGAGAACCGCAGGGAGG(+CAGTGT)GTACCACAGATGAGAGACAACGCTTCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605194352_l55b TCACAGATAGGTCCAAATAGCACTATCCCAAG(+CAGTGT)GATCCCACAAAAGAACTTGGGCATACTTATT NGG 10 8 17 8 24 0 100 5 10
    240605194351_g56y GTATCAATAAGTATGCCCAAGTTCTTTTGTGG(+CAGTGT)GATCCTTGGGATAGTGCTATTTGGACCTATC NGG 10 8 17 8 24 0 100 5 10
    240605194349_p8ue CAGCAGGCCTGGTAGCCCAGTGATGAGAAAGG(+CAGTGT)GATCCGCCCTTCCCTGTGGATGTATGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605194347_7xn9 GCCTTCTGCTCATACATCCACAGGGAAGGGCG(+CAGTGT)GATCCCTTTCTCATCACTGGGCTACCAGGCC NGG 10 8 17 8 24 0 100 5 10