Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194435_sv88 GACAGGAGCGCAGAGGTGAGAGAAAACAACAG(+CAGTGT)GATCCTCCCAAGAAGTCCTTTCCTTAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605194433_kz6g CACAAAGGCCTAAGGAAAGGACTTCTTGGGAG(+CAGTGT)GATCCTGTTGTTTTCTCTCACCTCTGCGCTC NGG 10 8 17 8 24 0 100 5 10
    240605194432_zrif GAGAATCCTGTTAAAATTGTTGGCTGTTACTG(+CAGTGT)GTACCCTATGTTAATCAGCCACCAGGTGTCA NGG 10 8 17 8 24 0 100 5 10
    240605194430_ltnl TGAAATGACACCTGGTGGCTGATTAACATAGG(+CAGTGT)GTACCAGTAACAGCCAACAATTTTAACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605194429_vtcq TGATACAGTCAGCACCAGCAGACCTGCTTCAG(+CAGTGT)GATCCAAGAAAGCTGGCTTTGTAGGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605194428_ms79 ACTGAAGACAGCCTACAAAGCCAGCTTTCTTG(+CAGTGT)GATCCTGAAGCAGGTCTGCTGGTGCTGACTG NGG 10 8 17 8 24 0 100 5 10
    240605194427_39nd CAGAACGAACCTTCTCTGGACTCAGATTTACG(+CAGTGT)GATCCCTGTCCATACCCGCTTCAGCTAACTA NGG 10 8 17 8 24 0 100 5 10
    240605194425_p2p4 AAGGCTAGTTAGCTGAAGCGGGTATGGACAGG(+CAGTGT)GATCCGTAAATCTGAGTCCAGAGAAGGTTCG NGG 10 8 17 8 24 0 100 5 10
    240605194424_nejo GTTAGGGTTAACGACACTGTTGTACTGTCATG(+CAGTGT)GTACCCCCTGTATAAAGGATCAGTTTGACTC NGG 10 8 17 8 24 0 100 5 10
    240605194422_fk73 AGCCAGAGTCAAACTGATCCTTTATACAGGGG(+CAGTGT)GTACCATGACAGTACAACAGTGTCGTTAACC NGG 10 8 17 8 24 0 100 5 10