Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194525_xa0y CTTTCATATTGCCACTAGGTCAGCCATTAAAG(+CAGTGT)GTACCACAAAATCACCTGTTTATGTGAATTA NGG 10 8 17 8 24 0 100 5 10
    240605194524_0za9 TTCATTAATTCACATAAACAGGTGATTTTGTG(+CAGTGT)GTACCTTTAATGGCTGACCTAGTGGCAATAT NGG 10 8 17 8 24 0 100 5 10
    240605194523_wjbt TTTCAGCCCCTTGTATCACTCTTCCTGATCTG(+CAGTGT)GTACCTGAGCAACCGCTGATCTGCTTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605194521_b2k1 TCAAGAGAAAAAGCAGATCAGCGGTTGCTCAG(+CAGTGT)GTACCAGATCAGGAAGAGTGATACAAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240605194520_zpt4 GGCCTTGCTTCACCACCCAAGCCAGCTGCTGG(+CAGTGT)GTACCCAGCCTGACTGCTGAAGTAAGACACT NGG 10 8 17 8 24 0 100 5 10
    240605194518_6y2w GCTGCAGTGTCTTACTTCAGCAGTCAGGCTGG(+CAGTGT)GTACCCAGCAGCTGGCTTGGGTGGTGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605194516_5ggo TAGAACAGGAAACGAGTAACATCTGGCCAAAG(+CAGTGT)GATCCCAACAGAAGGAACCTGATAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240605194515_7ess CTTTTTCATTTTTATCAGGTTCCTTCTGTTGG(+CAGTGT)GATCCTTTGGCCAGATGTTACTCGTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605194513_1t8o GAGCAGGGGCAAGCTCTCGGGCTGAAGGGTGG(+CAGTGT)GATCCACGCGTGGCTCGAGGACACAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605194511_xouo CCGGTTTCCCTTGTGTCCTCGAGCCACGCGTG(+CAGTGT)GATCCCACCCTTCAGCCCGAGAGCTTGCCCC NGG 10 8 17 8 24 0 100 5 10