Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194453_khi8 ACCCAGATGAAGGCTGCTTTTCTACATCATAG(+CAGTGT)GTACCCTTAGGGGCCAATAACTATATATTAG NGG 10 8 17 8 24 0 100 5 10
    240605194450_42yz CTATTCTAATATATAGTTATTGGCCCCTAAGG(+CAGTGT)GTACCTATGATGTAGAAAAGCAGCCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240605194448_aq8z TTCAATTCCCAGTACCCATGTCAGCTCTAGGG(+CAGTGT)GATCCAATGTTCTCTCCTGTCCTCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605194447_b68c GCACTGGTTCTAGAGGACAGGAGAGAACATTG(+CAGTGT)GATCCCCTAGAGCTGACATGGGTACTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240605194445_xc8s GTGTCTACTTCGTAAGAGCCGTGTCTATCCAG(+CAGTGT)GTACCAAAAGTGAGAGGTCAGCAGTGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605194444_p69h CCAGCCCACCCACTGCTGACCTCTCACTTTTG(+CAGTGT)GTACCTGGATAGACACGGCTCTTACGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605194442_dv89 CTGAGGACATCACGGAAGCCCAGAGATTAGGG(+CAGTGT)GTACCACTGGAGGAGCCTCACTGCAGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605194440_1a0v CTCCAGGCTCCTGCAGTGAGGCTCCTCCAGTG(+CAGTGT)GTACCCCTAATCTCTGGGCTTCCGTGATGTC NGG 10 8 17 8 24 0 100 5 10
    240605194438_m08t ATTGAAGCAGGGATGGCTTGTCATAGCAGTGG(+CAGTGT)GATCCAGCTTCACCTGAGTTACTCTACCAAA NGG 10 8 17 8 24 0 100 5 10
    240605194436_cys2 CTGTGTTTGGTAGAGTAACTCAGGTGAAGCTG(+CAGTGT)GATCCCACTGCTATGACAAGCCATCCCTGCT NGG 10 8 17 8 24 0 100 5 10