Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194540_wugk TAGTAATAGAACAGTGATTAAGACAAAAGTTG(+CAGTGT)GTACCAAGGAGCAAAGTATTCTATGAAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605194539_6jor GTTAGGCTTTTCATAGAATACTTTGCTCCTTG(+CAGTGT)GTACCAACTTTTGTCTTAATCACTGTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605194537_uq4r ATGGATTTCTTACTCCTCTTCTCCATGGCTGG(+CAGTGT)GATCCTAGCTTCAGTCCTCATATCTGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605194536_e5d2 GGTGAGTAAACAGATATGAGGACTGAAGCTAG(+CAGTGT)GATCCCAGCCATGGAGAAGAGGAGTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605194534_kf30 TGCACACATGACATCTATGTAGCATATGCTAG(+CAGTGT)GATCCAGACGGACCTTAGAGTCTAAATCACC NGG 10 8 17 8 24 0 100 5 10
    240605194533_23jf AGCATGGTGATTTAGACTCTAAGGTCCGTCTG(+CAGTGT)GATCCTAGCATATGCTACATAGATGTCATGT NGG 10 8 17 8 24 0 100 5 10
    240605194532_pn1k AGCACTTCACCTGCACGAAGGGATGAGACTGG(+CAGTGT)GATCCCTGAGTAAACTTCTTTAATAGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240605194530_6e15 ATAGCACACACTATTAAAGAAGTTTACTCAGG(+CAGTGT)GATCCCAGTCTCATCCCTTCGTGCAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605194529_vub5 CTGGTTCCCATGGAAGCCAGAAGAGGGTGCTG(+CAGTGT)GATCCCTTGGAACTTGAGTTACAGACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605194527_6ygu GGTTGCAGCTGTCTGTAACTCAAGTTCCAAGG(+CAGTGT)GATCCAGCACCCTCTTCTGGCTTCCATGGGA NGG 10 8 17 8 24 0 100 5 10