Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605194509_es43 AAGGAAATGGTGACCAGGATGTCGGGGTGGGG(+CAGTGT)GATCCACTTCTGCTTCTTGAGGGCTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605194507_7r70 CTCCCCAAGACAGCCCTCAAGAAGCAGAAGTG(+CAGTGT)GATCCCCCACCCCGACATCCTGGTCACCATT NGG 10 8 17 8 24 0 100 5 10
    240605194506_u58a CGAAGAGGACCCAGACCAAGAAGGCCAACCTG(+CAGTGT)GATCCATGAAATTCAGAGGCAATGAGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240605194504_v1jh TTAATTTAACCTCATTGCCTCTGAATTTCATG(+CAGTGT)GATCCAGGTTGGCCTTCTTGGTCTGGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605194502_6eeh CTAAACAAAGTGATGTGTATGAAGATAGCTGG(+CAGTGT)GATCCTTAGAATTCCTTCTTTTTTTCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605194500_mglr ATTTTTGAAAGAAAAAAAGAAGGAATTCTAAG(+CAGTGT)GATCCCAGCTATCTTCATACACATCACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605194458_v39l CATGCATTCCAAGGAAAAAGGATGGGTCAGAG(+CAGTGT)GATCCAATTCACTTTTAGGCTTCCGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240605194457_9ctb GTAAAACTGTCCGGAAGCCTAAAAGTGAATTG(+CAGTGT)GATCCTCTGACCCATCCTTTTTCCTTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605194456_2cbq TCATACATGCATACGAATAAATATATTTATAG(+CAGTGT)GATCCATTCCTTTAAAATACGAATAAATTTA NGG 10 8 17 8 24 0 100 5 10
    240605194454_r7d0 TCCTATAAATTTATTCGTATTTTAAAGGAATG(+CAGTGT)GATCCTATAAATATATTTATTCGTATGCATG NGG 10 8 17 8 24 0 100 5 10